Composite

Part:BBa_K3760001:Design

Designed by: Ha Buer   Group: iGEM21_LZU-CHINA   (2021-10-01)


6x His tag-Rfx Cas13d


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2422
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 235
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Adding a 6 x His tag to the 5' end of ACE2 gene for purification by Ni-NTA.

Source

Ruminococcus flavefaciens.

References

Konermann S, Lotfy P, Brideau NJ, Oki J, Shokhirev MN, Hsu PD. Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors. Cell. 2018 Apr 19;173(3):665-676.e14. doi: 10.1016/j.cell.2018.02.033. Epub 2018 Mar 15. PMID: 29551272; PMCID: PMC5910255.